Kinetics of the Reaction between Moringa oleifera Leaf Extracts and 2,2-diphenyl-1-picrylhydrazyl (DPPH) Free Radical


Journal article


Geniel Talavera, Kenia Martínez, Apolinar Picado, Juan Alonso
Nexo Revista Científica, vol. 36(1), 2023 Feb 22, pp. 17-25


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Cite

APA   Click to copy
Talavera, G., Martínez, K., Picado, A., & Alonso, J. (2023). Kinetics of the Reaction between Moringa oleifera Leaf Extracts and 2,2-diphenyl-1-picrylhydrazyl (DPPH) Free Radical. Nexo Revista Científica, 36(1), 17–25. https://doi.org/10.5377/nexo.v36i01.15792


Chicago/Turabian   Click to copy
Talavera, Geniel, Kenia Martínez, Apolinar Picado, and Juan Alonso. “Kinetics of the Reaction between Moringa Oleifera Leaf Extracts and 2,2-Diphenyl-1-Picrylhydrazyl (DPPH) Free Radical.” Nexo Revista Científica 36, no. 1 (February 22, 2023): 17–25.


MLA   Click to copy
Talavera, Geniel, et al. “Kinetics of the Reaction between Moringa Oleifera Leaf Extracts and 2,2-Diphenyl-1-Picrylhydrazyl (DPPH) Free Radical.” Nexo Revista Científica, vol. 36, no. 1, Feb. 2023, pp. 17–25, doi:10.5377/nexo.v36i01.15792.


BibTeX   Click to copy

@article{geniel2023a,
  title = {Kinetics of the Reaction between Moringa oleifera Leaf Extracts and 2,2-diphenyl-1-picrylhydrazyl (DPPH) Free Radical},
  year = {2023},
  month = feb,
  day = {22},
  issue = {1},
  journal = {Nexo Revista Científica},
  pages = {17-25},
  volume = {36},
  doi = {10.5377/nexo.v36i01.15792},
  author = {Talavera, Geniel and Martínez, Kenia and Picado, Apolinar and Alonso, Juan},
  month_numeric = {2}
}

In this study, an antioxidant-rich extract was obtained by leaching Moringa oleifera leaves. The activity of the extract against the 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical was measured by determining a second-order rate constant (k2). The reaction between the mixture of antioxidants in the extract and the DPPH was monitored by measuring the absorbance at 517 nm in 30-second intervals for a period of 600 s. The absorbance exhibited a rapid decay within the first 100 s, followed by a slower decay. The absorbance data were fitted to a bi-exponential model. The curve fitting showed a linear relationship between the initial concentration of antioxidants and the reaction rate with the coefficient of determination values greater than 0.9979. The k2 value was 0.0870 ± 0.0007 mg/mL.